Kay E Davies CBE, DBE, F.MedSci., FRS
|Fax (PA)||01865 285880|
|Contact address||MRC Functional Genomics Unit, Department of Physiology Anatomy & Genetics, University of Oxford, Parks Road, Oxford, OX1 3PT, United Kingdom|
Professor Kay Davies
Our research is focused on the understanding of muscle disease, movement and behavioural disorders. We have developed various genetic models in order to understand disease pathogenesis as well as to develop effective treatments. Our studies range from the analysis of single gene defects such as Duchenne muscular dystrophy (DMD) to the use of genetic models which provide insights into the understanding of neurodegenerative diseases such as ataxia and amytrophic lateral sclerosis (ALS), as well as mental impairment and schizophrenia.
Current Research Programmes
Duchenne muscular dystrophy is the most common genetic form of muscular dystrophy affecting 1 in 3000 boys. Although the disease is known to be caused by the absence of the cytoskeletal protein, dystrophin, no effective treatment is yet available. Any effective therapy has to replace the dystrophin-associated protein complex to reconstruct the vital link between the extracellular matrix and internal cytoskeleton.
The dystrophin-associated protein complex
Therapy for DMD is a challenge since the protein is large and needs to be delivered to many muscle cells in the body for therapeutic effect. However the field has reached an exciting phase where there are several strategies for therapy in clinical trials. We are taking a novel approach to treatment through the analysis of a protein closely related to dystrophin, called utrophin. We have demonstrated that over expression of utrophin in the mdx animal model of the disease prevents the muscle pathology in both skeletal and cardiac muscle. Our data suggest that up-regulation of utrophin might be an effective treatment. One of the lead compounds developed with Summit plc which modulates the expression of dystrophin is called SMT C1100. Summit plc have just successfully completed a Phase I trial and a Phase II trial is planned. We are working with them to determine the mechanism of action of SMT C1100 which will assist us in moving this drug into the clinic. With our colleagues in the Department of Chemistry (Professor Steve Davies and Dr Angela Russell), we are also screening for follow up next generation compounds using a new knock-in mouse model which screens the promotor region in its full genomic context. We currently have new hits which we are evaluating in the mdx mouse model.
Improved muscle pathology using SMT C1100
An additional promising strategy for DMD is the delivery of DNA vectors that cause the transcriptional machinery in muscle cells to miss out the mutated exons in dystrophin responsible for DMD (exon skipping); this method generates a smaller but functional protein that will prevent the disease occurring. Using antisense oligonucleotides into U7snRNA/AAV vectors, we have shown that this ‘exon skipping’ approach can successfully restore expression of the dystrophin gene in cellular and animal models of DMD. The advantage of AAV based U7snRNA vectors are that they show more sustained expression than naked antisense oligonucleotides. This work is being carried out in collaboration with Aurelie Goyenvalle and Luis Garcia in Versailles, France.
NEURODEGENERATIVE AND BEHAVIOURAL DISORDERS
This work is in collaboration with an ERC Fellow, Dr Peter Oliver. We are investigating movement and neurodegenerative disorders through the characterisation of specific neurological mutants from the ENU mutagenesis programme at the MRC Mammalian Genetics Unit at Harwell. This has included studying models of human ataxia (robotic and moonwalker mutants) and peripheral neuropathy (trembler mutants) that have lead to the identification of potential therapeutic strategies for these disorders.
Oxidative stress in the central nervous system is a feature of some of the most common neurodegenerative disorders such as Parkinson’s disease (PD), Huntington’s disease (HD) and ALS. We have recently shown that the loss of the Oxr1 gene in bella mutant causes ataxia and cell death in the cerebellum. The function of this gene in unknown; however we know it is part of the protective pathways that are induced in neurons under oxidative stress. Current work is establishing the function of Oxr1 using primary neuronal cell culture, protein interaction assays and transgenic mouse models. We are also looking at the expression of OXR1 and related genes in human neurodegenerative disease.
Cell death in the cerebellum of bella mutants
The blind-drunk mutant harbours a mutation in SNAP-25, a protein essential for normal exocytosis in neuronal cells. These mice display ataxia, but also a complex behavioural profile with aspects relevant to psychiatric diseases such as schizophrenia. Recently we have been investigating the influence of the environment on this behaviour. In addition, we are examining the sleep / wake (circadian) activity of these mice, as sleep disturbance is a common symptom of schizophrenia. Our data has shown that blind-drunk mutants show disrupted circadian behaviour, allowing us to examine common mechanisms that linking fundamental synaptic pathways to rest / activity profiles in mental health. This work is also funded by the Wellcome Trust Strategic Award led by Russell Foster in the department of Clinical Neurosciences and is part of the Oxford Sleep and Circadian Neuroscience Institute (SCNi).
Circadian disruption in the Bdr mutant
Within Oxford University:
Dr Peter Oliver (Physiology Anatomy and Genetics) on mouse models of neurological disease
Prof Steve Davies and Dr Angela Russell (Department of Chemistry) on compound screening
Dr Kieran Clarke (Physiology Anatomy and Genetics) on cardiac function in skeletal muscle mutants
Dr Zoltan Molnar (Physiology, Anatomy and Genetics) on schizophrenia, cortical development and non-coding RNA in the CNS
Prof Chris Ponting (FGU) on the function of non-coding RNA in the CNS
Prof David Bannerman (Experimental Psychology) on molecular basis of behaviour
Dr Kevin Talbot (Physiology, Anatomy and Genetics/Clinical Neurology) on motor neuron disease
Dr Maike Glitsch (Physiology, Anatomy and Genetics) on the electrophysiology of Trp channels
Prof Russell Foster (Department of Opthalmology) on circadian analysis of synaptic mouse mutants
Sheena Lee (OXION) on microarray expression analysis and pathway modeling
Prof Steve Brown and Dr Pat Nolan (MRC Mammalian Genetics Unit, Harwell, Oxon) on mouse neurological mutants
Dr Francesco Muntoni (Hammersmith Hospital, London) on muscular dystrophy
Prof David Sattelle (University of Manchester) on worm models of SMA.
Muscular Dystrophy Association, USA
Muscular Dystrophy Campaign, UK
Association Francaise contre les Myopathies, France
Medical Research Council, UK
- Dr Emmanuelle Bitoun, Postdoctoral Researcher
- Dr Rebecca Fairclough, Postdoctoral Researcher
- Dr Janet Kenyon, Postdoctoral Researcher
- Kelly Perkins, Postdoctoral Researcher
- Arran Babbs, Research Assistant
- Ben Edwards, Research Assistant
- Allyson Potter, Research Assistant
- David Powell, Research Assistant
- Sarah Squire, Research Assistant
- Anna Dulneva, Graduate Student
- Achilleas Livieratos, Graduate Student
- Kevin Liu, Graduate Student
- Leigh Paton, Graduate Student
- James Sleigh, Graduate Student
- Yixing Wu, Graduate Student